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Old August 15th, 2010, 11:35 PM   #1
Mean519
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Live well additives - best?

Which live well additive do you use/like best to keep fish healthy until weigh-in? Rejuvenate...Please Release Me...U2...Catch and Release? Heard some like to mix it in water bottles, freeze 'em, then put them in the well with the cap off to keep the temps down and slowly release the additive as the ice melts.
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Old August 16th, 2010, 02:34 AM   #2
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I use rejuvenate. I haven't lost any fish using it and my fish are usually full of life when I pull them out of the well
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Old August 16th, 2010, 06:56 AM   #3
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I use rejuvenate. I haven't lost any fish using it and my fish are usually full of life when I pull them out of the well
I do the same.+1
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Old August 16th, 2010, 07:14 AM   #4
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U2 is the best product out there, Please release me and Rejuvenade are not liquids and takes awhile to dissolve and they also have a shelf life of 1 yr. If you run any kind of Oxygenator system Please Release me and Rejuvenade will harm your units also.
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Old August 16th, 2010, 07:17 AM   #5
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I do the same.+1
+2 rejuvenate; its like fish crack....
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Old August 16th, 2010, 07:33 AM   #6
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Old August 16th, 2010, 11:05 AM   #7
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U2

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Originally Posted by Chris Love View Post
U2 is the best product out there, Please release me and Rejuvenade are not liquids and takes awhile to dissolve and they also have a shelf life of 1 yr. If you run any kind of Oxygenator system Please Release me and Rejuvenade will harm your units also.
+++ U2 Fresh Water Pro Formula is the best IMHO. I have had issues with Rejuvenade my self. U2 is very similar to the stuff they use to transport live fish to market. It is easy on the fish and not harmful to humans who consume their fish. Since I switched over, and I also use the Oxygenator Live Well System, I have not lost a fish and when I take the fish out of the livewell they are in good shape. 1/2 ounce will treat a 30 gallon livewell.
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Old August 16th, 2010, 11:08 AM   #8
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Rejuvenate for the most part. This weekend I tried hydrogen peroxide because I have been hearing that it helps a ton. Bullshit. I weighed my first dead fish and the others were not far behind. I'm not sure if the rejuvenate countered with the hydro and hurt them or people are full of shit. I'm going with the full of shit.
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Old August 16th, 2010, 12:00 PM   #9
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U2 is the best product out there, Please release me and Rejuvenade are not liquids and takes awhile to dissolve and they also have a shelf life of 1 yr. If you run any kind of Oxygenator system Please Release me and Rejuvenade will harm your units also.
That's correct on the 1 yr shelf life. A fishing buddy from CA had all of his fish die one tournament as he had a bottle close to 2-3 years old (sitting in the sun, etc). He learned the hard way about shelf life of this product. Just be sure to use the recommended dosage and keep those pumps on all the time during the warmer months. Bring ice too as it helps considerably.
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Old August 16th, 2010, 12:14 PM   #10
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No additives and haven't ever weighed in a dead fish..
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Old August 16th, 2010, 01:00 PM   #11
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No additives and haven't ever weighed in a dead fish..
You probably should catch one before you chime in. Just a thought
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Old August 16th, 2010, 01:48 PM   #12
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Old August 16th, 2010, 02:56 PM   #13
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watching a fishing show the other day, two universities (florida and georgia I think) and one private firm did studies and couldn't find any definative evidence that any of those live well products work. Just FYI for ya fellas.
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Old August 16th, 2010, 06:45 PM   #14
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Hydrogen peroxide. 1 ounce to 6 gallons.
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Old August 18th, 2010, 03:36 AM   #15
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Here's some experts' opinion along with some powerful scientific references and fish physiological documentation without the typical sales BS, shucking and jiving, smoke and mirrors... marketing magic:

Salt or Oxygen?
Given this choice, what's best for taking stress out of fish?

By Adam Johnson

Fighting, handling and holding fish in captivity place severe metabolic demands on brain, muscle, heart, gill and other organ tissues putting them at considerable physiological risk. In general terms we call this stress, but the physiological situation is highly complicated. The degree of stress fish realize, and the potential for subsequent recovery, depends on the type and duration of the physiological stress we place them in and the environment in which they are allowed to recover. To gain a better understanding of fishing-related stress, we must first gain a basic understanding of some of the physiological mechanisms involved.

Energy Metabolism - A Continued Need for Oxygen

The energy used to fuel virtually all cellular functions in every living thing is derived from the compound adenosine triphosphate, or ATP. ATP is needed to make muscles contract, drive brain impulses, allow the heart to beat, provide oxygen uptake by the gills and on an on. ATP is made up of adenosine (A) attached to three phosphate groups (triphosphate - TP). When the cell needs energy the last phosphate bond is broken and chemical energy is released. The cell converts this chemical energy into the mechanical energy needed to perform the work of the cell.

The by-products remaining after this reaction are adenosine diphosphate (ADP) and inorganic phosphate (Pi). In the cell, ADP and Pi can again recombine through a series of complicated metabolic pathways to re-form ATP, and the energy cycle continues. To biochemists, the metabolic reaction looks like this:

ATP ADP + Pi

Most freshwater fish rely heavily on oxygen in their environment. This oxygen is used, primarily, to help fuel the biochemical mechanisms associated with the energy recycling processes. Oxygen-associated energy metabolism is highly efficient, and produces the constant supply of energy that fish (or people, for that matter) rely upon to support basically all physiological functions. As long as oxygen and food (fuel) are readily available, the recycling of energy continues unimpeded and the energy supply meets demand. Energy metabolism using oxygen is called "aerobic" metabolism.

Not all energy production relies on oxygen, however. Cells have developed mechanisms to maintain energy supply during short bursts of sudden, high-intensity exercise, or for short periods when oxygen levels are inadequate (a situation known as hypoxia, or lack of oxygen). Anaerobic or hypoxic energy metabolism is inefficient and cannot be relied on to produce enough energy to keep tissues for very long. To keep a steady balance between energy utilization and supply, fish need oxygen.

Fish must rely on constant supplies of energy. And to get the energy they need, fish also must rely on constant and plentiful supplies of oxygen. Lack of oxygen will quickly deprive fish of the energy they need to sustain life.

Catching Fish Depletes Energy Reserves

Fish can swim continuously for long distances without tiring at a broad range of speeds. This type of swimming, called steady state swimming, is used by fish during normal cruising, or for long distance travel. Muscles that are used in this type of exercise use high volumes of oxygen for energy synthesis. As long as there is a constant supply of oxygen, fish basically never become tired during this type of exercise.

Sudden bursts of high-intensity swimming are called burst swimming. This type of swimming normally lasts for only seconds (possibly minutes) and ends in a physical state of exhaustion.(1,2) Burst swimming is critical when fish attack prey, when they migrate against strong currents or up waterfalls, or when they are fighting after being hooked. This high-intensity exercise totally drains fish of energy reserves. Recovery from such exhaustive exercise may take hours, or sometimes days, depending on the availability of oxygen following the exercise, the duration of the exercise and the degree to which energy compounds are consumed by, or lost from, the fish's tissue. Energy metabolism during burst swimming is anaerobic, providing only enough energy for a few seconds. If the exercise continues, tissue energy stores will become completely drained.(1)

Think of this in terms of a sprint-type exercise over a 100-yard dash. When you sprint your leg muscles totally consume their energy in only seconds. Short rest between sprints allows the muscle to restore a small amount of energy, but the next sprint is harder and slower. With continued exercise sprinting becomes continually difficult until the muscle is totally exhausted and you cannot run another step. Muscles become weak and spongy, and if you are not used to the exercise they will be sore for several days. Only the oxygen you breath after the exercise will allow the energy in your leg muscles to recover, reducing the soreness and regaining muscle strength. Imagine trying to recover if a plastic bag was pulled over your head!

Now relate this example to a fish involving its entire body in an all-consuming sprint-type exercise lasting 30-seconds, two minutes or longer. Energy from the whole body is recruited and used up. Even in a well-oxygenated environment, like a trout stream, the fish will need to find a quiet place to rest for several hours before it regains its energy. Imagine this fish placed in a livewell with little or no oxygen. Energy cannot recover and the fish will either die, or become so energy starved it will likely die later. It is not the lack of oxygen that kills the fish. It is the lack of energy and the inability to recover lost energy stores.

Factors Affecting Recovery

Associated with the depletion of energy reserves during burst swimming is an increase in tissue (including blood) lactic acid (or lactate). As an acid, lactate produces hydrogen ions that lower the pH of the tissue, ultimately reducing the total energy supply of the cell.(1,3) It also drains the cell of important metabolites it needs to recover. Once these metabolites are exhausted, the fish will not be able to perform another burst of exercise until they are replenished. Clearance of lactic acid, and restoration of normal cellular function, can take anywhere from four to 12 hours. Over this time the fish is able to restore lost metabolites, but 12-hours still may not be sufficiently long to allow cellular energy levels to rebound. Factors such as body size, water temperature, water hardness, water pH and oxygen availability all play a part in time to recovery.

The following is a list summarizing the effects of certain factors on the physiology and recovery from exhaustive exercise in fish:

- Body size: There is a positive correlation between anaerobic energy metabolism and power requirements of burst exercise in the rainbow trout. In general, bigger fish require more relative energy to perform burst swimming exercise. This creates a larger drain on energy reserves, taking longer to recover.(4)
- Environmental temperature: Clearance rate of lactic acid and energy-draining metabolites are significantly affected by acclimation to temperature. Large changes in ambient temperature dramatically effect the fish's ability to recover.(5) Dramatic heating or cooling of the environment will reduce recovery rate.
- Water hardness: A reduction in the hardness of environmental water has a minimal, but important, effect on the metabolic and acid-base status of the blood.(3) Much of the work describing this effect has been conducted in saltwater species, so it is not fully known if the results are directly transferable to freshwater fish. What is known, however, is that when freshwater fish are stressed, water flows across cell membranes (particularly those of the gills) and the blood becomes diluted. This dilution puts additional pressure on maintaining salt balance in the fish. Maintaining salt balance is called osmoregulation and it will be described in more detail later.
- Water pH: Moderate water acidity will help fish recover more quickly. Higher water pH will slow the recovery process dramatically.(3)

Why are these things important? Stress associated with catch and release can contribute to catch related mortality. The lessons learned from studies investigating the affects of, and recovery from, exhaustive exercise have the potential to decrease the number of stress-related deaths and increase fish productivity. Understanding the energy metabolism of fish, and the factors that affect energy metabolism, are critical to understanding how fish must be handled and treated when caught.

Osmoregulation - Maintaining Salt Balance in Stressed Fish

The regulation of salt (ion) balance is fundamental to all life. The structure and function of cells depend closely on their interactions with water and things that are dissolved in water, and few factors affect the viability of an organism as extensively as osmoregulation. Thus, fish invest considerable energy in controlling the composition of intracellular and extracellular fluids. In fish, osmoregulation typically consumes 25% - 50% of the total metabolic energy output, possibly the largest energy consumer in the animal.(6,7,8)

The mechanisms used by fish to maintain salt balance are highly complicated and extremely energy dependent. Since anaerobic energy metabolism is less than 1/10 as efficient as energy metabolism in an oxygen rich environment, the energy demand of tissue osmoregulation cannot be met by anaerobic energy metabolism alone. A rapid fall in cellular ATP levels causes a slow-down, and eventual stop, in the cellular pumps used to control the movement of salts across the cell membrane. A disruption ion pump activity causes the cell to lose ion homeostasis, and ions are then free to run down their concentration gradients putting the survival of the cell - and the fish - at risk.

Both fresh- and saltwater fish are constantly faced with the challenge of ionic and osmotic regulation. Freshwater fish, in which tissue ion concentrations are much greater than the water in their surrounding environment, must deal with osmotic water uptake and loss of ions through permeable epithelial tissues and via the urine. The opposite is obviously true in saltwater fish. Freshwater fish produce copious amounts of highly dilute urine. In fact, they will produce urine weighing up to 20% of their body weight every day. Imagine a 200-pound man generating 40-pounds - or about five gallons - of urine every day! While fish kidneys are highly efficient at removing water from the fish, they are equally efficient at keeping body salts out of the urine. This mechanism allows the salts to stay in the body, helping to control salt balance. While very small amounts of salt are passed in the urine, most osmoregulation is managed by cells in the fish's gills.

Sodium is the primary ion found in tissue. Transport of sodium across cell membranes is highly energy dependent and is facilitated by an enzyme called Na/K-ATPase. This enzyme resides right inside the cell membrane and uses the energy supplied by ATP to move sodium in one direction across the cell membrane, while its counter-ion (potassium or whatever) moves in the other direction. This process allows muscles to contract, it provides the electrochemical gradient needed to stimulate the heartbeat, and allows all manner of brain and nerve signals to be transmitted.(9,10)

Imagine yourself to be a Na/K ATPase enzyme in line in a fast food restaurant. You hand your money to the attendant, pick up your change, and then grab your number three value meal. The same is true in the fish's cell. A sodium ion is handed across the cell membrane, a second (different) ion is picked up, and this ion is brought back into the cell.

Most osmoregulation occurs in the fish's gill. The enzyme Na/K-ATPase is primarily responsible for maintaining salt balance and it resides in the membrane of the fish gill cell. There is one pump on the side of the gill cell next to the blood, and another in the membrane on the side next to the water. In freshwater fish, the system of osmoregulation works like this…

Ammonia is produced as a waste product of fish metabolism. When fish exercise, they produce a great deal of ammonia, and that ammonia has to be excreted from the blood. Unlike higher animals, fish do not excrete ammonia in the urine. Instead, ammonia (and most all other nitrogen waste) diffuses through the gill membrane. About 80% - 90% of the nitrogenous waste of fish metabolism are excreted via the gills.

Ammonia from the blood is exchanged across the gill cell membrane for sodium. This removes the ammonia from the blood and increases the ammonia concentration in the gill cell. In turn, a sodium ion is passed from the gill cell and into the blood. The plasma sodium concentration is increased and the sodium concentration in the gill cell goes down. To replace the sodium ion in the gill cell and restore its salt balance the cell then passes the ammonia out of the gill cell into the environment and exchanges it for another sodium ion from the water.

In like fashion, chloride ions from the water are exchanged for bicarbonate. Bicarbonate is formed when CO2 from cellular respiration combines with water in the cell. Remember, like us, fish use oxygen in respiration. The byproducts of respiration are CO2 and water. We get rid of CO2 by simply exhaling, but obviously fish can't do that. Instead, the CO2 formed in fish respiration combines with water in the cell to form bicarbonate ion. Chloride ions move into the cell and bicarbonate moves out of the cell and into the environment.(11) Hydrogen ions can also be exchanged for sodium in this manner, helping to control blood pH. For a graphic depiction of all this refer to Figure 1.

Figure 1: Diagram of the model of ion transport in the fish gill
(modified after several authors)



These two mechanisms for ion exchange are called absorption and secretion and occur within two cell types of the fish gill known as respiratory cells and chloride cells. Given that chloride cells are used to eliminate salts, they are larger and more highly developed in saltwater species and not well established in freshwater species. Respiratory cells, which are involved in gas exchange, nitrogenous waste removal and acid-base balance, are more developed in freshwater fish. They are supplied by arterial flow and are used to exchange sodium and chloride for ammonia and bicarbonate, respectively.

Again, the most important point to remember is that the exchange of ions that takes place in these cells is highly dependent on energy availability. If there is not enough energy to drive the ion pumps, these exchanges cannot occur and water will flood the cells by diffusion, killing the fish. Maintaining control of salt and water balance is vital and requires considerable metabolic energy to power it. Energy is the key constituent.

Consequences of Oxygen Starvation on Osmoregulation

After only a few minutes of oxygen starvation the membrane surrounding brain cells loses it ability to control ion balance causing a release of chemicals (called neurotransmitters) that speed up calcium influx into the cell. The increased level of calcium in the cells triggers a number of degenerative processes that can lead to neurological damage or death. These processes involve the destruction of DNA, important cellular proteins, and even the cell membrane itself. Free radicals and nitric oxide are also formed, eating away further at the cell membrane and attacking intracellular constituents.(12,13) The important events of this catastrophe are common to most vertebrate brains, including both fish and humans. Similar events occur in other tissues, like liver(14-16), muscle(7), blood cells(17) and heart(9). Once calcium has invaded the cells it takes much more energy to remove it, via the ATP-dependent calcium pumps.

Another consequence of oxygen starvation (or hypoxia) is the release of hormones from the pituitary gland. Prolactin is the most prevalent of these hormones in most fish species, both fresh- and saltwater. The release of this hormone affects the permeability of cell membranes in the gill, skin, kidney, intestine and urinary bladder and impacts ion transport mechanisms. Prolactin release helps regulate water-ion balance by decreasing water uptake and promoting retention of important ions (especially Na+ and Cl-). In doing so, prolactin helps to maintain the salt balance of the blood and tissues and keeps the fish from swelling with water.(11)

The main threat to freshwater fish is the loss of ions by diffusion into the external environment rather than the elimination of excess water. Even though the regulation of water balance may be important, it is secondary to the importance of ion retention. However, the effect of prolactin on water permeability should not be dismissed as inconsequential. Prolactin decreases the osmotic permeability of the gills, retaining ions and excluding water. It also increases gill mucous secretion, contributing to ion-water balance by impeding the passage of molecules across the membrane.

Salt or Oxygen?

So, which is more important, salt or oxygen? The answer is clear. In fish that have been stressed by sudden bursts of high-intensity exercise - like fighting at the end of a line - energy deprivation is the most vital concern. Tissues become almost totally depleted of energy, and it takes several hours (or perhaps days) for them to recover. Anaerobic energy metabolism cannot keep pace with cellular demand and large amounts of oxygen are needed to drive the pathways of energy recovery in the cell. Oxygen deprivation will not allow these pathways to function efficiently, if at all. And the result is dead fish. They might not die right away, but they will die. Salt balance, no matter how much salt there is in the livewell environment, cannot be maintained without large amounts energy to fuel the process. And while the importance of maintaining ion balance cannot be over emphasized, the first consideration must be providing stressed fish the energy they need to turn on the osmoregulatory processes.

How Much Oxygen is Enough?

Oxygen, not water temperature or salt level, is the main culprit in fish death in the livewell or in catch-related stress. However, livewell water temperature is a main determinant of how much oxygen can be made available to fish and how quickly they will utilize what's available.

The maximum amount of dissolved oxygen in water is called its saturation level. Saturation level decrease as the temperature of the water increases. For example, at 70 degrees, water saturates at 8.9 parts per million (ppm). At 80 degrees, saturation is achieved at 8.0 ppm, and at 90 degrees only 7.3 ppm. At higher temperatures, fish metabolism also increases and they use oxygen faster. Therefore, at 80 degrees, oxygen concentrations below 5.0 ppm may prove quickly fatal.

Here is an example used by Hal Schramm, noted fisheries biologist, that will put this temperature/metabolism/oxygen relationship into perspective. Ten pounds of bass in a 15-gallon livewell will reduce the oxygen concentration from 75% saturation to stress levels in about eight minutes at 60 degrees; in seven minutes at 70 degrees; and in only 2.5 minutes at 85 degrees.(18)

Standard livewell aeration systems simply cannot keep up with this oxygen demand. A recirculating aeration system will raise the oxygen level in a 15-gallon livewell from 3 ppm to 7 ppm in about eight minutes when the water is 60 degrees. It will take about 14 minutes at 70 degrees. At 85 degrees, a standard livewell system simply cannot get to 7 ppm. With several fish in the livewell, a standard livewell system is not able to keep the oxygen level above stressful limits that may prove fatal, or will certainly create stress on the fish that may not be recoverable - almost certainly leading to delayed fish mortality.

Decreasing the water temperature with ice is one solution, but remember that too great a change in water temperature adds its own element of stress. Large changes in water temperature affect lactic acid clearance and slow metabolic recovery. In addition, to lower the water temperature by five degrees for a full tournament day in temperatures above 85 degrees could require up to 50-pounds of ice! Relying on ice to sufficiently cool a livewell to fully oxygenate the water is unrealistic.

Supplemental oxygen is required, along with temperature control of the livewell, to supply fish the oxygen they need to recover from metabolic stress and promote osmoregulation. There are no two ways about it; oxygen delivery is the key to helping fish overcome the stress that comes with angling and survival in the livewell.

References:

1. Milligan CL. Metabolic recovery from exhaustive exercise in rainbow trout: Review. Comp Biochem Physiol, 1996; 113A:51-60.
2. Moyes CD, TG West. Exercise metabolism in fish. In: Biochemistry and Molecular Biology of Fishes, Volume 4 (Eds. Hochachka and Mommsen). Elsevier Science, 1995, Boston.
3. Rossiter AM. Physiology and survival of Atlantic salmon following exhaustive exercise in soft and acidic water: implications for the catch and release fishery. M.Sc. Thesis. 1996. Queen's University, Kingston, Canada. 86pp.
4. Ferguson RA, JD Kieffer, BL Tufts. The effects of body size on the acid-base and metabolic status in the white muscle of rainbow trout before and after exhaustive exercise. J Exp Biol, 1993; 180:195-207.
5. Kiefer JD, S Currie, BL Tufts. Effects of environmental temperature on the metabolic and acid-base responses on rainbow trout to exhaustive exercise. J Exp Biol, 1994; 194:299-317.
6. Grau EG. Environmental physiology and comparative endocrinology of estuarine fish. Available on-line www2.hawaii.edu/zoology/graduate/faculty/grau.htm.
7. Laiz-Carrion R, S Sangiao-Alvarellos, JM Guzman, MP Martin, JM Miguez, JL Soengas, JM Mancera. Energy metabolism in fish tissues relaed to osmoregulation and cortisol action: Fish growth and metabolism. Environmental, nutritional and hormonal regulation. Fish Physiol and Biochem, 2002; 27(3-4):179-188.
8. Morgan JD, GK Iwama. Energy cost of NaCl transport in isolated gills of cutthroat trout. Am J Physiol, 1999; 277(3 Pt 2):R631-639.
9. MacCormack TJ, WR Driedzic. Mitochondrial ATP-sensitive K+ channels influence force development and anoxic contractility in a flatfish, yellowtail flounder Limanda ferruginea, but not Atlantic cod Gadus morhua heart. J Exp Biol, 2002; 205:1411-1418.
10. Slagle R. Gill Na,K-ATPase and osmoregulation in the sailfin molly, Poecilia latipinna. Honor's Thesis, 1986. Lafayette College, Easton, Pa.
11. Manzon LA. The role of prolactin in fish osmoregulation: a review. Gen Compar Endocrin, 2002; 125:291-310.
12. Nilsson GE, M Perez-Pinzon, K Dimberg, S Winberg. Brain sensitivity to anoxia in fish as reflected by changes in extracellular potassium-ion activity. Am J Physiol, 1993; 264:R250-R253.
13. Hylland P, GE Nilsson, D Johansson. Anoxic brain failure in an ectothermic vertebrate: release of amino acids and K+ in rainbow trout thalamus. Am J Physiol, 1995; 269:R1077-R1084.
14. Krumschnabel G, PJ Schwarzbaum, J Lisch, C Biasi, W Weiser. Oxygen-dependent energetics of anoxia-intolerant hepatocytes. J Mol Biol, 2000; 203(Pt 5):951-959.
15. Krumschnabel G, C Biasi, W Weiser. Action of adenosine on energetics, protein synthesis and K(+) homeostasis in teleost hepatocytes. J Exp Biol, 2000; 203(Pt 27):2657-2665.
16. Krumschnabel G, C Manzl, PJ Schwartzbaum. Importance of glycolysis for the energetics of anoxia-tolerant and anoxia-intolerant teleost hepatocytes. Physiol Biochem Zool, 2001; 74(3):413-419.
17. Pesquero J, T Roig, J Bermudez, J Sanchez. Energy metabolism by trout red blood cells: substrate utilization. J Exp Biol, 1994; 193:183-190.
18. Schramm H. Surviving the summer. Guest article, Bassmaster online. Available at espn.go.com/outdoors/bassmaster/s/bass_biology_surviving_summer.html.

Oxygen systems that deliver pure oxygen must deliver enough pure oxygen continuously to satisfy the cellular oxygen debt and cellular demand for all the bait or fish in the livewell that are in crisis. Any oxygen system that delivers a fixed or limited flow of oxygen may easily deliver less oxygen than a good aerator or water pump which is often deadly every summer. Know and understand the limitations of any pure oxygen system, it's important. Without enough oxygen supplied continuously, all the salt, chemicals, ice and any other life saving efforts will fail.

http://www.oxyedge-chum.com/oxygen_or_salt.htm

An opportunity to learn something new about what your trying to do with your bass can be valuable, some times.
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